hek293t atcc crl Search Results


transient transfection hek293tδp1 cells  (ATCC)
99
ATCC transient transfection hek293tδp1 cells
Fig. 3 | Evaluation for loss-of-function effect of <t>Piezo1</t> PBS mutations in <t>HEK293TΔP1</t> cells. a Representative confocal images (n = 12) of HEK293TΔP1 cells co- expressing WT-tdTom with either S260R-GFP (upper) or S2211L-GFP (lower) fused proteins. b Single-channel current recordings of WT and PBS variant proteins expressing WT-tdTom alone, WT-tdTom with either S260R-GFP or S2211L-GFP. WT- GFP with S2211L-GFP was used as control. c 30 s all-point current histograms of the single-channel recordings shown in (b). d Steady state NPo of WT-dtTom and PBS- GFP variants. Statistical analysis was performed by using One-way ANOVA where ***p < 0.001, ****p < 0.0001 and ns = not significant. tdTom = tdTomato, C = close, O = open, pA = picoamperes, NC = normalized counts, NPo = normalized open probability, calibration bar = 10 µm. Data points in (d) are represented as Mean ± SD.
Transient Transfection Hek293tδp1 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hek293t+atcc+crl/Piezo1+knock-out+HEK293T/pm38184690-254-36-47
Average 99 stars, based on 1 article reviews
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hek293t 17 cells  (ATCC)
99
ATCC hek293t 17 cells
Fig. 3 | Evaluation for loss-of-function effect of <t>Piezo1</t> PBS mutations in <t>HEK293TΔP1</t> cells. a Representative confocal images (n = 12) of HEK293TΔP1 cells co- expressing WT-tdTom with either S260R-GFP (upper) or S2211L-GFP (lower) fused proteins. b Single-channel current recordings of WT and PBS variant proteins expressing WT-tdTom alone, WT-tdTom with either S260R-GFP or S2211L-GFP. WT- GFP with S2211L-GFP was used as control. c 30 s all-point current histograms of the single-channel recordings shown in (b). d Steady state NPo of WT-dtTom and PBS- GFP variants. Statistical analysis was performed by using One-way ANOVA where ***p < 0.001, ****p < 0.0001 and ns = not significant. tdTom = tdTomato, C = close, O = open, pA = picoamperes, NC = normalized counts, NPo = normalized open probability, calibration bar = 10 µm. Data points in (d) are represented as Mean ± SD.
Hek293t 17 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hek293t+atcc+crl/OAT1+HEK+293T%2F17/10__1096_slash_fj__201801766rrr-58-1-4
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human oat1  (ATCC)
94
ATCC human oat1
Fig. 3 | Evaluation for loss-of-function effect of <t>Piezo1</t> PBS mutations in <t>HEK293TΔP1</t> cells. a Representative confocal images (n = 12) of HEK293TΔP1 cells co- expressing WT-tdTom with either S260R-GFP (upper) or S2211L-GFP (lower) fused proteins. b Single-channel current recordings of WT and PBS variant proteins expressing WT-tdTom alone, WT-tdTom with either S260R-GFP or S2211L-GFP. WT- GFP with S2211L-GFP was used as control. c 30 s all-point current histograms of the single-channel recordings shown in (b). d Steady state NPo of WT-dtTom and PBS- GFP variants. Statistical analysis was performed by using One-way ANOVA where ***p < 0.001, ****p < 0.0001 and ns = not significant. tdTom = tdTomato, C = close, O = open, pA = picoamperes, NC = normalized counts, NPo = normalized open probability, calibration bar = 10 µm. Data points in (d) are represented as Mean ± SD.
Human Oat1, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hek293t+atcc+crl/OAT1%3B+HEK+293T%2F17%3B+Human/pm41391530-49-13-20
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virus strains a549 atcc ccl 185 mdck ii atcc crl 2936 hek293t atcc crl 3216 hek293t hace 2 bei resources nr52511 vero e6 nccs pune  (Microbiologics Inc)
86
Microbiologics Inc virus strains a549 atcc ccl 185 mdck ii atcc crl 2936 hek293t atcc crl 3216 hek293t hace 2 bei resources nr52511 vero e6 nccs pune
Fig. 3 | Evaluation for loss-of-function effect of <t>Piezo1</t> PBS mutations in <t>HEK293TΔP1</t> cells. a Representative confocal images (n = 12) of HEK293TΔP1 cells co- expressing WT-tdTom with either S260R-GFP (upper) or S2211L-GFP (lower) fused proteins. b Single-channel current recordings of WT and PBS variant proteins expressing WT-tdTom alone, WT-tdTom with either S260R-GFP or S2211L-GFP. WT- GFP with S2211L-GFP was used as control. c 30 s all-point current histograms of the single-channel recordings shown in (b). d Steady state NPo of WT-dtTom and PBS- GFP variants. Statistical analysis was performed by using One-way ANOVA where ***p < 0.001, ****p < 0.0001 and ns = not significant. tdTom = tdTomato, C = close, O = open, pA = picoamperes, NC = normalized counts, NPo = normalized open probability, calibration bar = 10 µm. Data points in (d) are represented as Mean ± SD.
Virus Strains A549 Atcc Ccl 185 Mdck Ii Atcc Crl 2936 Hek293t Atcc Crl 3216 Hek293t Hace 2 Bei Resources Nr52511 Vero E6 Nccs Pune, supplied by Microbiologics Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hek293t+atcc+crl/185+2+2936+3216+a549+atcc+atcc+atcc+bei+ccl+crl+crl+e6+hace+hek293t+hek293t+ii+mdck+nccs+nr52511+pune+resources+strains+vero+virus/ppr0553017-536-5-28
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Image Search Results


Fig. 3 | Evaluation for loss-of-function effect of Piezo1 PBS mutations in HEK293TΔP1 cells. a Representative confocal images (n = 12) of HEK293TΔP1 cells co- expressing WT-tdTom with either S260R-GFP (upper) or S2211L-GFP (lower) fused proteins. b Single-channel current recordings of WT and PBS variant proteins expressing WT-tdTom alone, WT-tdTom with either S260R-GFP or S2211L-GFP. WT- GFP with S2211L-GFP was used as control. c 30 s all-point current histograms of the single-channel recordings shown in (b). d Steady state NPo of WT-dtTom and PBS- GFP variants. Statistical analysis was performed by using One-way ANOVA where ***p < 0.001, ****p < 0.0001 and ns = not significant. tdTom = tdTomato, C = close, O = open, pA = picoamperes, NC = normalized counts, NPo = normalized open probability, calibration bar = 10 µm. Data points in (d) are represented as Mean ± SD.

Journal: Nature communications

Article Title: PIEZO1 loss-of-function compound heterozygous mutations in the rare congenital human disorder Prune Belly Syndrome.

doi: 10.1038/s41467-023-44594-0

Figure Lengend Snippet: Fig. 3 | Evaluation for loss-of-function effect of Piezo1 PBS mutations in HEK293TΔP1 cells. a Representative confocal images (n = 12) of HEK293TΔP1 cells co- expressing WT-tdTom with either S260R-GFP (upper) or S2211L-GFP (lower) fused proteins. b Single-channel current recordings of WT and PBS variant proteins expressing WT-tdTom alone, WT-tdTom with either S260R-GFP or S2211L-GFP. WT- GFP with S2211L-GFP was used as control. c 30 s all-point current histograms of the single-channel recordings shown in (b). d Steady state NPo of WT-dtTom and PBS- GFP variants. Statistical analysis was performed by using One-way ANOVA where ***p < 0.001, ****p < 0.0001 and ns = not significant. tdTom = tdTomato, C = close, O = open, pA = picoamperes, NC = normalized counts, NPo = normalized open probability, calibration bar = 10 µm. Data points in (d) are represented as Mean ± SD.

Article Snippet: Primers for sequencing were purchased from IDT and are as follows: S260R (forward):5’-CTGGTGGTCCTGTCACTTTC-3’ S260R (reverse):5’-GCTCTGGCTGGTTAGTACAT-3’ S2211L (forward):5’-AGCCGAGAGACAGAGAAGAA-3’ S2211L (reverse):5’-CTCAGGACTGTACTGGCTAATG-3’ C-term w/2422-Myc tag (forward): 5’-TTCCCCATCTCTTCCCC AAG-3’ C-term w/2422-Myc tag (reverse): 5’- GGAAGATGAGCTTGGC GTATAG −3’ Cell culture and transient transfection HEK293TΔP1 cells (PIEZO1 Knockout cell line) were purchased from ATCC (#CRL-3519).

Techniques: Expressing, Variant Assay, Control